Recombinant Human CD24 (mFc Tag)

Description :
FAQ COA 已发表文献 CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa 60 kDa GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance . CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs. 产品性质 别名 CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043 Uniprot No. P25063.1 Recombinant Human CD24Protein is expressed from HEK293Cellswith mFc tag at the C-terminal. It contains Ser27-Gly59. 分子量 Approximately 29.5 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData: Immobilized Human CD24, mFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 0.19g/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.