Recombinant Human CD209/DC-SIGN (His Tag)

Description :
FAQ COA 已发表文献 Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family. The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein. The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain. Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms. DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs. DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation. 产品性质 别名 CD209; CLEC4L; DC-SIGN; DC-SIGN1; CDSIGN; MGC129965; CD209 molecule Uniprot No. Q9NNX6.1 Recombinant Human CD209/DC-SIGNProtein is expressed from HEK293Cellswith His tag at the N-terminal. It contains Gln59-Ala404. 分子量 Approximately 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. SPRData: Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 M as determined in SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.