Recombinant Human BTN3A2 (His-Avi Tag)

Description :
FAQ COA 已发表文献 BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region with one V-type Ig-like domain and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. A rodent counterpart to BTN3A2 has not been reported. BTN3A2 mRNA overexpression was associated with a good prognosis in relation to disease-free and overall survival in a cohort of 55 epithelial ovarian cancer (EOC) patients . Another study in a larger cohort of 199 high-grade EOC patients further confirmed that the protein expression of BTN3A2 in ovarian cancer tissues is positively correlated with the intraepithelial infiltration of CD4+ and CD8+ T cells, suggesting that BTN3A2 was a co-stimulatory molecule to modulate the infiltration of immune cells and thus the anti-cancer immunity. 产品性质 别名 BT3.2; BTF3; BTF4; BT3.3; BTN3A2; FLJ40011; BTN3.2; CD277 Uniprot No. P78410.1 Recombinant Human BTN3A2Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gln30-Trp248. 分子量 Approximately 26.5 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.