Recombinant Human LILRA5/CD85f/ILT11 (His-Avi Tag)

Description :
FAQ COA 已发表文献 The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs). Human LILRA5, also known as ILT11, LIR-9, and CD85f, consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail. The ECD contains two Ig-like domains, and the transmembrane segment contains a positively charged aspartic acid residue which may mediate its association with the signaling molecule, FcR common gamma chain . Alternative splicing generates additional isoforms with a 12 aa deletion within the signal peptide and/or a 27 aa substitution for the C-terminal 61 residues. This substitution encompasses both the transmembrane and cytoplasmic regions. LILRA5 is expressed by monocytes, macrophages, and neutrophils. It is found as an approximately 40 kDa molecule on the cell surface, while a soluble 25 kDa form can be released into the synovial fluid of rheumatoid arthritis patients. Cross-linking of LILRA5 on monocytes induces the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) as well as the anti-inflammatory IL-10. 产品性质 别名 CD85; CD85f; ILT11; ILT-11; ILT11CD85f; LILRA5; LILRB7; LIR9; LIR-9 Uniprot No. A6NI73.1 Recombinant Human LILRA5/CD85f/ILT11Protein is expressed from HEK293Cells with His tag and Avi tag at the C-terminal. It contains Gly42-Arg268. 分子量 Approximately 28.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.