Recombinant Human LILRA2/CD85h/ILT1 (His-Avi Tag)

Description :
FAQ COA 已发表文献 LILRA2, also known as ILT1, CD85h, and LIR7, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA2 consists of a 426 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Alternative splicing generates isoforms with short deletions between the fourth Ig-like domain and the transmembrane region, and an isoform that is truncated C-terminal to the fourth Ig-like domain. LILRA2 is expressed on monocytes, neutrophils, basophils, and eosinophils. It contains a positively charged arginine residue in its transmembrane segment, enabling association with the signaling protein Fc epsilon RI gamma. Cross-linking of LILRA2 on monocytes induces the production of multiple cytokines as well as the upregulation of Fc gamma receptors. 产品性质 别名 CD85h; ILT1; ILT-1; ILT1CD85H; LILRA2; LIR7 Uniprot No. Q8N149.1 Recombinant Human LILRA1/CD85i/LIR-6Protein is expressed from HEK293Cells with His tag and Avi tag at the C-terminal. It contains Gly24-Asn449. 分子量 Approximately 49.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.