Recombinant Human BTN1A1/Butyrophilin (His-Avi Tag)

Description :
FAQ COA 已发表文献 Butyrophilin 1A1 (also called BTN1A1), a 55 kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 494 amino acids (aa) long and is composed of an extracellular domain (ECD) (aa 27-242), a transmembrane domain and a cytoplasmic tail (aa 270-526) which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members.The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion. Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1. The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma T cells. In vitro, BTN1A1 has an inhibitory effect on CD4+ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma. 产品性质 别名 BT; BTN1A1; BTN; Butyrophilin Uniprot No. Q13410 Recombinant Human BTN1A1/ButyrophilinProtein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Ala27-Arg242. 分子量 Approximately 26.9 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.