Recombinant Human DNAM-1/CD226 (His-Avi Tag)

Description :
FAQ COA 已发表文献 DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts. Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung. CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation. In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation. 产品性质 别名 CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 Uniprot No. Q15762 Recombinant Human DNAM-1/CD226Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Glu19-Asn247. 分子量 Approximately 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. SPR DataHuman CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22M as determined in a SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.