Recombinant Human CD160 (His-Avi Tag)

Description :
FAQ COA 已发表文献 CD160 (also Natural killer cell receptor BY55) is a 27-30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. These include CD56DIMCD16+cytolytic NK cells, CD8+CD28-T cells, CD8+CD101+IELs, NKT cells, gamma TCR T cells, activated CD4+T cells, and vascular endothelial cells. CD160 was initially identified as a GPIlinked glycoprotein. It is synthesized as a preproprotein that is 181 amino acids (aa) in length. The precursor contains a 26 aa signal sequence, a 133 aa mature molecule that shows one 96 aa V-type Ig-like domain (aa 27-122), and a 22 aa prosegment that is cleaved to generate a GPI-linkage at Ser159. GPI-linked CD160 is known to be cleaved by phospholipases and these generate an 80 kDa (presumably trimeric) band in SDS-PAGE. Alternative splice forms for CD160 are reported to exist on activated NK cells. The principal variant is an extended type I transmembrane (TM) protein that shows a 55 aa substitution for the C-terminal two amino acids. It contains a 23 aa TM segment (aa 160-182) and a 52 aa cytoplasmic region. Two other variants show deletions of the Ig-like domain in both the GPI-linked and TM form . 产品性质 别名 CD160; BY55; BY55FLJ46513; NK1; NK28 Uniprot No. O95971 Recombinant Human CD160Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gly25-Leu158. 分子量 Approximately 17.7 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. SPR Data: Human HVEM captured on Protein A chip, can bind Human CD160, His Tag with an affinity constant of 1.2M as determined in a SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.