Recombinant Human Fc gamma RIIIA/CD16a (V176) (His-Avi Tag)

Description :
FAQ COA 已发表文献 Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta. Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA. Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity. Shedding from monocytes and macrophages can be trIgGered by cell activation or phagocytosis. Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases. 产品性质 别名 Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20 Accession AAH17865 Recombinant Human Fc gamma RIIIA/CD16a (V176)Protein is expressed from HEK293Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208. 分子量 Approximately 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human Fc gamma RIIIA (V176) , His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09g/ml determined by ELISA. SPR DataHuman Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.