Recombinant Human Fc gamma RIIA/CD32a(R167) (His-Avi Tag)

Description :
FAQ COA 已发表文献 Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16). Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified. The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. 产品性质 别名 Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A Uniprot No. P12318.1 Recombinant Human Fc gamma RIIA/CD32a (R167)Protein is expressed from HEK293Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218. 分子量 Approximately 23.2 kDa. Due to glycosylation, the protein migrates to 34-40 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. SPR DataHuman Fc gamma RIIA (R167) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 9M as determined in SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.