Recombinant Human CD3E & CD3D/CD3 epsilon & CD3 delta (hFc Tag)

Description :
FAQ COA 已发表文献 T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3d , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3d, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. 产品性质 别名 CD3; CD3e; CD3E; CD3d; T3D; CD3d; CD3E&CD3d; CD3 delta&CD3 epsilon Uniprot No. P07766(CD3E)&P04234(CD3d) Recombinant Human CD3E&CD3d/CD3 epsilon&CD3 deltaProtein is expressed from HEK293Cells with hFc at the C-terminal. It contains Asp23 – Asp126 (CD3E) & Phe22 – Ala105(CD3d). 分子量 Approximately 37.8 kDa (CD3E) and 35.4 kDa (CD3d). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA Data: Immobilized Human CD3E&CD3d, hFc Tag at 0.5g/ml (100l/Well). Dose response curve for Biotinylated Anti-CD3E&CD3d Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA. ELISA Data: Immobilized Human CD3E&CD3d, hFc Tag at 0.1g/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3d Ab.2, mFc Tag with the EC50 of 27.0ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.