Recombinant Human CD3E/CD3 epsilon (His Tag)

Description :
FAQ COA 已发表文献 CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a type I transmembrane protein that belongs to the Ig (Immunoglobulin) superfamily. Human CD3 epsilon consists of 104 amino acid (aa) extracellular domain, 26 aa transmembrane domain and 55 aa cytoplasmic domain. There are four CD3 proteins: CD3, CD3 epsilon, CD3 gamma, and CD3 zeta. All CD3 proteins contain ITAM (Immunoreceptor Tyrosine-based Activation Motifs) in the cytoplasmic tail, which becomes phosphorylated by Src family protein tyrosine kinases LCK and FYN upon TCR engagement. CD3 proteins form heterodimers of CD3/CD3 epsilon and CD3 gamma /CD3 epsilon, which bind to TCR and form trimeric TCR alpha /CD3 epsilon /CD3 gamma and TCR beta /CD3 gamma /CD3 epsilon. The resulting heterohexamer further associates with CD3 zeta homodimer and forms TCR/CD3 signaling complex. Similar complex is also formed with TCR gamma and TCR. CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency). 产品性质 别名 CD3e; CD3e; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 Uniprot No. P07766 Recombinant Human CD3e/CD3 epsilonProtein is expressed from HEK293Cells with His tag at the C-terminal. It contains Asp23-Asp126. 分子量 Approximately 12.6 kDa. Due to glycosylation, the protein migrates to 15 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGE. ELISA Data: Immobilized Human CD3e, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.