Recombinant Human ACE2/ACEH (His Tag)

Description :
FAQ COA 已发表文献 Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE. ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues. ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo, functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters. Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury, heart disease, liver and lung fibrosis, inflammatory lung disease, and cardiopulmonary disease . Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail. The N-terminal peptidase region is required for binding to SARS-CoV and SARS-CoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters. The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity. 产品性质 别名 ACE-2; ACEH; ACE2 Uniprot No. Q9BYF1.1 Recombinant Human ACE2/ACEH Protein is expressed from HEK293Cellswith His tag at the C-terminal. It contains Gln18-Ser740. 分子量 Approximately 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. SPRData: SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.