Recombinant Human Sigle3/CD33(hFc Tag)

Description :
FAQ COA 已发表文献 Human Siglec-3 is alternatively known as myeloid cell surface antigen CD33 and GP67.To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. Siglec-3 expression is restricted to cells of myelomonocytic lineage. 产品性质 别名Synonyms CD33 molecule, CD33, FLJ00391, gp67, Siglec3, Siglec-3, p67 Uniprot No. P20138-1 Source Recombinant Human Siglec-3 /CD33 Protein is expressed from HEK293Cellswith hFctag at the C-terminal. It contains Asp18-His259. 分子量(Molecular Weight) Approximately 53.6kDa.Due to glycosylation, the protein migrates to68-80kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human Siglec-3 at 0.5g/mL(100L/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50of 33.9ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.