Recombinant Human NKG2D/CD314(His-Avi Tag)

Description :
FAQ COA 已发表文献 NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. Human NKG2D is expressed on CD8+alpha beta T cells, gamma T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection. 产品性质 别名Synonyms CD314, D12S2489E, KLR, NKG2-D, NKG2D Uniprot No. P26718 Source Recombinant Human NKG2D / CD314 Protein is expressed from HEK293 Cells with His tag and Avi tag at the N-terminal. It contains Phe78-Val216. 分子量(Molecular Weight) Approximately 19kDa. Due to glycosylation, the protein migrates to36-38kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity ELISAData: Immobilized Human NKG2D, His Tag at 1g/mL(100L/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50of 4.7ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.