Recombinant Human EGFR/HER1 Protein, hFc Tag

Description :
FAQ COA 已发表文献 The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR , ErbB2,ErbB3, and ErbB4. All family members are type I transmembrane glycoproteins.EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, and HB-EGF. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs. 产品性质 别名 ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 Uniprot No. P00533 Recombinant Human EGFR/HER1Protein is expressed from HEK293Cells with hFc tag at the C-terminal. It contains Leu25-Ser645. 分子量 Approximately 95.2 kDa. Due to glycosylation, the protein migrates to 140-160 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human EGF at 2g/ml (100l/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.