Recombinant Human Nectin-3/CD113 Protein,His-Avi Tag

Description :
FAQ COA 已发表文献 The Nectin family has at least four members (Nectin-1 – 4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectin-3 activity adds strength to the junction through trans-interactions with a variety of molecules, including Nectin-3 itself, as well as Nectins-1 and 2 plus Necl-1, 2 and 5. 产品性质 别名Synonyms CD113, CDw113, FLJ90624, PPR3, PVRL3, PVRR3, PRR3 Uniprot No. Q9NQS3 Source Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly58-Asp400. 分子量(Molecular Weight) Approximately 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity 1.SPRData: Human TIGIT captured on Protein A chip, can bind Human Nectin-3, His Tag with an affinity constant of 44M as determined in a SPR assay (Biacore T200). . 2.SPRData: Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 M as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.