Recombinant Human LILRB3/CD85a/ILT5(His-Avi Tag)

Description :
FAQ COA 已发表文献 Leukocyte immunoglobulin-like receptor subfamilyB (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors. Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells. LILRB3 can also bind to ligands exposed on necrotic tumor cells. 产品性质 别名Synonyms CD85a, ILT5, ILT-5, LILRB3, LIR3, LIR3CD85A, LIR-3MGC138403, PIRB, HL9 Uniprot No. O75022-1 Source Recombinant Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gly24-Glu443. 分子量(Molecular Weight) Approximately 50kDa.Due to glycosylation, the protein migrates to60-72kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human LILRB3, His Tag at 5g/mL(100L/well) on the plate. Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50of 27.3ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.