Recombinant Human FLT3/Flk-2(His-Avi Tag)

Description :
FAQ COA 已发表文献 The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a 130-155 kDa member of the classIII subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Within ECD human Flt-3 shares 85%and 82%aa sequence identity with mouse and rat Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. 产品性质 别名Synonyms CD135, FLK2, STK-1, Flt-3 Uniprot No. P36888-1 Source Recombinant Human FLT3/Flk-2 Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal.It containsAsn27-Asn541. 分子量(Molecular Weight) Approximately 61.2kDa.Due to glycosylation, the protein migrates to80-105kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human FLT3, His Tag at 0.5g/mL(100L/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50of 0.1g/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.