Recombinant Human Fc gamma RIIIB/CD16b (NA1)(His Tag)

Description :
FAQ COA 已发表文献 Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and Bshare 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NKcells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes. 产品性质 别名Synonyms CD16, FCG3, FCGR3B, FCRIIIB, IGFR3, CD16b (NA2), FCG3B, FCG3, FCGR3, FCR-10 Uniprot No. AAA35881.1 Source Recombinant Human Fc gamma RIIIB / CD16b (NA1) Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gly17-Ser200. 分子量(Molecular Weight) Approximately 22kDa. Due to glycosylation, the protein migrates to48-55kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity SPRData: Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIIB (NA1) , His Tag with an affinity constant of 0.117 M as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.