Recombinant Human Survivin(HLA-A*02:01)(His-Avi Tag)

Description :
FAQ COA 已发表文献 Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.Normally it is only expressed in actively proliferating cells, but is upregulated in most, if not all cancers; consequently, it has received significant attention as a potential oncotherapeutic target. 产品性质 别名Synonyms Survivin, MHC, MHC I, BIRC5, API4, EPR-1, IAP4, survivin variant 3 alpha Uniprot No. A0A140T913(HLA-A*02:01)&P61769(B2M)&LMLGEFLKL Source Recombinant Human Survivin(HLA-A*02:01) Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and LMLGEFLKL peptide. 分子量(Molecular Weight) Approximately 50.5kDa.Due to glycosylation, the protein migrates to53-60kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity Testing in progress 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.