Recombinant Human GP100(HLA-A*02:01)(His-Avi Tag)

Description :
FAQ COA 已发表文献 Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100. 产品性质 别名Synonyms HLA0201, MHC I, gp100, Glycoprotein 100, ME20-M, P100 Uniprot No. A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA Source Recombinant Human GP100(HLA-A*02:01)Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide. 分子量(Molecular Weight) Approximately 50.4kDa.Due to glycosylation, the protein migrates to52-62kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human GP100 (HLA-A*02:01) Complex, His Tag at 2g/mL(100L/Well) on the plate. Dose response curve for Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag with the EC50of 32.9ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.