Recombinant Human FOLR1(His-Avi Tag)

Description :
FAQ COA 已发表文献 Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 – 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which diein uterowith gross morphological defects. 产品性质 别名Synonyms FR-alpha, FR alpha, FR, FOLR, FBP, Folbp1, KB cells FBP, MOv18, Fbp1, Folate receptor alpha Uniprot No. P15328 Source RecombinantHuman FOLR1Protein is expressed fromHEK293Cellswith His tag and Avi tag at the C-terminal. It contains Arg25-Met233. 分子量(Molecular Weight) Approximately27.5kDa.Due to glycosylation, the protein migrates to38-45kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% as determined by SDS-PAGEandHPLC Activity ELISAData: Immobilized Human FOLR1, His Tag at 0.5g/mL(100L/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50of 11.9ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EUper1g oftheprotein bytheLAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.