Recombinant Human AFP(HLA-A*02:01)(His-Avi Tag)

Description :
FAQ COA 已发表文献 AFP (alpha-Fetoprotein), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. 产品性质 别名Synonyms Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-feto, HPAFP, AFPD, FETA, MHC Uniprot No. A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI Source Recombinant Human AFP(HLA-A*02:01)Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal.It containsGly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide. 分子量(Molecular Weight) Approximately 50.6kDa.Due to glycosylation, the protein migrates to53-60kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human AFP (HLA-A*02:01) Complex, His Tag at 2g/mL(100L/well) on the plate. Dose response curve for Anti-AFP (HLA-A*02:01) Antibody, hFc Tag with the EC50of 8.2ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.