Recombinant Human VEGFR2/KDR(mFc Tag)

Description :
FAQ COA 已发表文献 VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist. 产品性质 别名Synonyms CD309, KDR, VEGFR, VEGFR2, VEGFR-21, FLK1, KRD1, Ly73 Uniprot No. P35968-1 Source Recombinant Human VEGF R2/KDR Protein is expressed from HEK293Cellswith mFc tag at the C-terminal. It contains Ala20-Glu764. 分子量(Molecular Weight) Approximately 110kDa.Due to glycosylation, the protein migrates to150-200kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human VEGF165 1g/mL(100L/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50of 36.1ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.