Recombinant Human Tim-3/HAVCR2(His Tag)

Description :
FAQ COA 已发表文献 TIM-3, also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA. 产品性质 别名Synonyms CD366, TIM3, TIM-3, TIMD-3, TIM 3, FLJ14428, HAVCR2, KIM3 Uniprot No. Q8TDQ0-1 Source HEK293 Cells-derivedHuman Tim-3/HAVCR2Protein, Ser22-Arg200with Histagat the C-terminal 分子量(Molecular Weight) Approximately 20.7kDa.Due to glycosylation, the protein migrates to 50-60kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human TIM-3, His Tag at 0.5g/mL(100L/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50of 8.6ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.