Recombinant Human TIGIT Protein, His Tag

Description :
FAQ COA 已发表文献 TIGIT, also called Vstm3, Vsig9 and WUCAM is a 30-34 kDa typeI transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. Within the ECD, human TIGIT shares only 65% and 67% aa sequence identity with mouse and rat TIGIT. It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium. Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production. Ligation of TIGIT on Tcells downregulates TCR-mediated activation and subsequent proliferation, while in NKcell, TIGIT ligation blocks NKcell cytotoxicity. In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1. Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates Tcell responses, while mice lacking TIGIT show increased Tcell responses and susceptibility to autoimmune challenges. 产品性质 别名Synonyms TIGIT, VSIG9, VSTM3, WUCAM Uniprot No. Q495A1-1 Source HEK293 Cells-derivedHuman TIGITProtein, Met22-Pro141 with Histagat the C-terminal 分子量(Molecular Weight) Approximately 14kDa.Due to glycosylation, the protein migrates to 20-25kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISAData: Immobilized Human TIGIT, His Tag at 0.5g/mL(100L/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50of 18ng/mLdetermined by ELISA. 2.SPRData: Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17M as determined in a SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.