Recombinant Human CD2/SRBC(His Tag)

Description :
FAQ COA 已发表文献 Tcell surface antigen CD2 is a typeI glycoprotein belonging to the Ig superfamily and is expressed on Tcells, NK cells, Bcells and some antigen presenting cells. CD2 functions as an activator of Tlymphocytes and thymocytes. Mature human CD2 consists of a 185amino acid (aa) extracellular domain (ECD), a 26aa transmembrane segment, and a 116aa cytoplasmic tail. The extracellular domain is composed of two immunoglobulin-superfamily domains with highly-charged binding regions. Within the ECD, human CD2 shares 47% and 48% aa sequence identity with mouse and rat CD2, respectively. It interacts with both CD58 and CD59 directly to activate Tcells and their adhesion pathways. The conformational flexibility of the CD2 molecule affects function through the conformational status of the adhesive ligands. Together with PSTPIP1, CD2 works to regulate Tcell activation. These two proteins colocalize with a second CD2-binding protein to signal immunological synapse formation in Tcells. CD2 is an adhesion molecule present on the cell surface of T cells, natural killer (NK) cells, and Bcells; and its interaction with CD58 on antigen-presenting cells plays an important role in their immune reaction. CD2-CD58 interactions play a critical role in the anti-tumor immune response, and restoration of this signaling is an important strategy for anti-tumor therapy. 产品性质 别名Synonyms T11; CD2; SRBC; LFA-2; LFA-3 receptor Uniprot No. P06729 Source Recombinant Human CD2/SRBC Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Lys25-Asp209. 分子量(Molecular Weight) The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity SPRDataHuman CD58, hFc Tag captured on CM5 Chip via Protein A can bind Human CD2, His Tag with an affinity constant of 440 nM as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.