Recombinant Human CD19(His Tag)

Description :
FAQ COA 已发表文献 CD19 protein is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19. It is required for theresponsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation. CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production. CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers. 产品性质 别名Synonyms CD19, B4, CVID3, Leu-12, MGC12802 Uniprot No. P15391-1 Source Recombinant Human CD19Protein is expressed from HEK293Cellswith Histag at the C-terminal. It contains Pro20-Lys291. 分子量(Molecular Weight) Approximately 34.7kDa.Due to glycosylation, the protein migrates to50-60kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity ELISAData: Immobilized Human CD19 Protein at 5g/mLcan bind different Anti-CD19 Antibodies with high affinity. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.