Recombinant Human CD48/SLAMF2(His Tag)

Description :
FAQ COA 已发表文献 CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily proteins. The human CD48 cDNA encodes a 243 amino acid (aa) precursor that includes a 26aa signal sequence, a 194 aa mature protein containing two Ig-like C2-type domains, and a 23 aa C-terminal propeptide. A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients. Human CD48 shares approximately 50% aa sequence identity with mouse and rat CD48. It shares 20%-26% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC. CD2, 2B4, and heparan sulfate function as CD48 ligands. CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4. CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells. High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation. CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor. 产品性质 别名Synonyms CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1; mCD48; MEM-102; TCT.1 Uniprot No. P09326-1 Source Recombinant Human CD48/SLAMF2 Protein is expressed from HEK293 Cellswith His tag at the C-terminal. It contains Gln27-Ser220. 分子量(Molecular Weight) The protein has a predicted MW of 23.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA DataImmobilized Human CD48, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50of 0.45g/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.