Recombinant Human IL-18BP Protein,hFc Tag

Description :
Source Recombinant Human IL-18BP Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Thr31-Gly194.[uniprot |O95998-2] Molecular Weight The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human IL-18 at 5g/ml (100l/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 36.5ng/ml determined by ELISA SPR Data: Human IL-18BP, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.