Recombinant Human CD47(His Tag)

Description :
FAQ COA 已发表文献 CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily . Human CD47 is an integral membrane protein that consists of a 123amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 34aa C-terminal cytoplasmic tail. Alternate splicing of human CD47 generates additional isoforms with deletions in the cytoplasmic tail. Within the N-terminal ECD, human CD47 shares 63%aasequence identity with mouse and rat CD47. A portion of the Nterminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium. The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection. 产品性质 别名Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 Uniprot No. Q08722-1 Source Recombinant Human CD47 is expressed from HEK293 Cellswith His tag at the C-terminal. It contains Gln19-Pro139. 分子量(Molecular Weight) The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA DataImmobilized Human CD47, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 28.9ng/ml determined by ELISA. 2.SPR DataHuman CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Sirpa, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.