Recombinant Human CD40/TNFRSF5(His Tag)

Description :
FAQ COA 已发表文献 CD40 Ligand, also known as TNFSF5, CD154, TRAP, or gp39, is a 33-39 kDa type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 aa cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. The extracellular domain of mouse CD40 Ligand shares 75% and 93% aa sequence identity with the human and rat proteins, respectively. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells. The 18 kDa soluble form (aa 112-260) arises from proteolytic processing and retains the ability to bind and activate CD40. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. 产品性质 别名Synonyms CD40; CD40L receptor; TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013; Uniprot No. P25942-1 Source Recombinant Human CD40/TNFRSF5 Protein is expressed from HEK293 Cellswith His tag at the C-terminal. It contains Glu21-Arg193. 分子量(Molecular Weight) The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC. Activity 1.ELISA Data: Immobilized Human CD40, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 0.13g/ml determined by ELISA. 2.ELISA Data: Immobilized Human CD40, His Tag at 0.2g/ml (100l/Well). Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50of 5.8ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.