Recombinant Human MSLN/Mesothelin(hFc Tag)

Description :
FAQ COA 已发表文献 Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. This recombinant human Mesothelin lacks the 8 aa insertion, and within aa 296-580 it shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. 产品性质 别名Synonyms MSLN, CAK1, Mesothelin, MPF, MPFSMRP, SMR Uniprot No. Q13421-2 Source HEK293 Cells-derivedHuman MSLN/Mesothelin Protein, Glu296-Asp580 with hFctag at the C-terminal 分子量(Molecular Weight) Approximately 59.0kDa.Due to glycosylation, the protein migrates to 68-78kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISAData: Immobilized Human CA125, His Tag at 2g/mL(100L/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50of 2.8g/mLdetermined by ELISA. 2.ELISAData: Immobilized Human MSLN at 0.5g/mL(100L/well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50of 29.2ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.