Recombinant Human CEACAM-5/CD66e(His-Avi Tag)

Description :
FAQ COA 已发表文献 CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species. Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor. CEACAM-5 is synthesized as a 180kDa, variably glycosylated molecule of which approximately 60%is carbohydrate. CEACAM-5 functions as acalcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1. CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors. This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors and is a commonly used cancer marker. It promotes tumor cell migration, invasion, adhesion, and metastasis. It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis). 产品性质 别名Synonyms CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392 Uniprot No. Q8N4D0 Source Recombinant Human CEACAM-5/CD66e Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Lys35-Ala685. 分子量(Molecular Weight) The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-150 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA DataImmobilized Human CEACAM-5 at 0.5g/ml (100l/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50of 14.8ng/ml determined by ELISA. 2.SPR Data: Human CEACAM-5, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CEACAM5 Antibody, hFc Tag with an affinity constant of 0.12nM as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.