Recombinant Human CD4/LEU3(hFc Tag)

Description :
FAQ COA 已发表文献 CD4, also known as L3T4, T4, and W3/25, is an approximately 55kDa typeI transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature Tlymphocytes. It is a standard phenotype marker for the identification of Tcell populations. Mature human CD4 consists of a 371aa extracellular region containing four immunoglobulin-like domains, a 22aa transmembrane segment, and a 40aa cytoplasmic domain. Within the ECD, human CD4 shares approximately 52%aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive Tcells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+or CD8+mature Tcells. CD4+SP cells, also known as Thelper cells, further differentiate into multiple subsets of CD4+cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity. CD4 is reexpressed on circulating CD8+Tcells upon activation and contributes to their cytotoxic effector activity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NKcells, and neurons and glial cells in the brain. Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse. CD4 binds directly to MHC classII molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. 产品性质 别名Synonyms CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 Uniprot No. P01730 Source Recombinant Human CD4/LEU3 Protein is expressed from HEK293 Cellswith hFc at the C-terminal. It contains Lys26-Trp390. 分子量(Molecular Weight) The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA DataImmobilized Human CD4 at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50of 20.9ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.