Recombinant Human PD-1/PDCD1(His Tag)

Description :
FAQ COA 已发表文献 Programmed Death-1 receptor, also known as PD-1 and CD279, is a receptor expressed on T cells responsible for modulating T cell activation. Like CTLA_x005f_x001e_4, PD-1 is classified as an immune checkpoint inhibitory receptor.Mature human PD-1 consists of an extracellular region (ECD) with one immunoglobulin-like V_x005f_x001e_type domain, a transmembrane domain, and a cytoplasmic region. The mature ECD of human PD-1 shares 61% amino acid sequence identity with mouse PD-1 ECD. 产品性质 别名Synonyms PDCD1, PD1, CD279, SLEB2 Uniprot No. Q15116-1 Source HEK293 Cells-derived Human PD-1 / PDCD1 Protein, Leu25-Gln167 with His tag at the C-terminal 分子量(Molecular Weight) Approximately 17.0 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISAData: Immobilized Human PD-1, His Tag at 0.5g/mL(100L/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50of 45.0ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.