Recombinant Human Beta Klotho Protein, His Tag

Description :
FAQ COA 已发表文献 Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase and sterol 12-alpha hydroxylase 产品性质 Synonyms betaKlotho; beta-klotho; BKL;KLB; klotho beta like; Klotho beta; -klotho; klotho like; Klotho Uniprot No. Q86Z14 Source HEK293 Cells-derivedHuman Beta Klotho Protein, Met30-Thr983with His tag at the C-terminal Molecular Weight Approximately 111.6kDa.Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.