Recombinant Human B7-H3/CD276 Protein, His Tag

Description :
FAQ COA 已发表文献 B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses. Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% aa sequence identity. 产品性质 Synonyms B7H3; B7-H3; CD276; PSEC0249;UNQ309; PRO352; B7 homolog 3; CD276 Uniprot No. Q5ZPR3-2 Source HEK293 Cells-derivedHuman B7-H3/CD276 Protein,Leu29-Pro245with His tag at the C-terminal Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA DataImmobilized Human B7-H3, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50of 29.4ng/ml determined by ELISA. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.