Recombinant Human OX40/TNFRSF4/CD134(hFc Tag)

Description :
FAQ COA 已发表文献 OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells.Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression. 产品性质 别名Synonyms CD134, OX40L receptor, TNFRSF4, ACT-135, Ly-70, OX40 homologue, TXGP1L, IMD16 Uniprot No. P43489 Source HEK293 Cells-derived Human OX40 / TNFRSF4 / CD134 Protein, Leu29-Ala216 with hFc tag at the C-terminal 分子量(Molecular Weight) Approximately 46.8kDa. Due to glycosylation, the protein migrates to 70-78kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity ELISAData: Immobilized Human OX40 Ligand (Trimer) , His Tag at 1g/mL(100L/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50of 0.27g/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.