Recombinant Human LAG3/CD223(His Tag)

Description :
FAQ COA 已发表文献 LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function.LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling. Human LAG-3 shares 70%, 67%, 76%, and 73% aa sequence identity with mouse, rat, porcine, and bovine LAG-3, respectively.LAG-3 is expressed on activated CD4+and CD8+T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells. LAG-3 on activated CD4+CD25+Tregcells plays a role in their suppressive activity. LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli. 产品性质 别名Synonyms CD223,FDC,LAG3,LAG-3,sLAG-3 Uniprot No. P18627-1 Source HEK293 Cells-derivedHuman LAG3 / CD223 Protein, Leu23-Gly434with His tag at the C-terminal 分子量(Molecular Weight) Approximately 45.5kDa.Due to glycosylation, the protein migrates to 55-65kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA Data: Immobilized Human LAG3, His Tag at 0.5g/ml (100L/Well). Dose response curve for Anti-LAG3 Ab., hFc Tag with the EC50of 0.17g/mLdetermined by ELISA. 2.ELISA Data: Immobilized Human LAG3, His Tag at 1g/mL(100L/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50of 2.3g/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.