Recombinant Human ICOS/CD278(His Tag)

Description :
FAQ COA 已发表文献 Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors.Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity.ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. 产品性质 别名Synonyms AILIM,CD278,CRP-1,CVID1,ICOS,MGC39850 Uniprot No. Q9Y6W8 Source HEK293 Cells-derivedHuman ICOS/CD278 Protein, Glu21-Gln134with His tag at the C-terminal 分子量(Molecular Weight) Approximately 14.6kDa.Due to glycosylation, the protein migrates to 25-35kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data: Immobilized Human ICOS, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50of 22.7ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.