Recombinant Human CD155/PVR(hFc Tag)

Description :
FAQ COA 已发表文献 CD155, also known as PVR (poliovirus receptor), is a 70kDa typeI transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily. CD155 binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically. Mature human CD155 consists of a 323aa extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24aa transmembrane segment, and a 50aa cytoplasmic tail. Within the ECD, human CD155 shares 45% aa sequence identity with mouse and rat CD155, and 52% with human Nectin-2. The V-type domain of CD155 mediates all binding, including to polio virus, and alternative splicing within this domain in humans can modulate ligand binding. Human CD155 can also be spliced to generate secreted isoforms. CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin. It is preferentially expressed on Th17 cells compared to Th2 cells, and its activation promotes the development of Th1 responses. On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration. Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells. 产品性质 别名Synonyms CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946 Uniprot No. P15151-1 Source Recombinant Human CD155/PVR Protein is expressed from HEK293 Cellswith hFc tag at the C-terminal. It contains Trp21-Asn343. 分子量(Molecular Weight) The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISA Data:Immobilized Human TIGIT, His Tag at 0.5g/ml (100l/Well). Dose response curve for Human CD155, hFc Tag with the EC50 of 0.18g/ml determined by ELISA. 2.SPR Data:Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 M as determined in SPR assay. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.