Recombinant Human B7-H5/Gi24/VISTA(His Tag)

Description :
FAQ COA 已发表文献 VISTA (V-domain immunoglobulin suppressor of T cell activation) is an immune checkpoint receptor expressed primarily on the surface of hematopoietic cells. Specifically, VISTA is expressed on nave CD4+ T cells and regulatory T cells. And it is up regulated in vivo on activated monocytes and dendritic cells. VISTA inhibits CD4+ and CD8+ T cell proliferation, and their production of the proinflammatory cytokines IL 2 and IFN gamma. VISTA protein is also observed on the surface of cancer cells and attenuates the anti tumor immune response enabling tumor progression. In the autoimmune disease model EAE, VISTA limits disease progression by downregulating the overactive immune system. VISTA, also known as B7 H5, PD-1H, Platelet receptor Gi24, Dies1, and SISP1, is a 55 65 kDa transmembrane glycoprotein with homology to B7 like immune co stimulatory molecules. VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP 4 induced signaling in ESC but is also down regulated following BMP 4 exposure . It binds to BMP 4 directly and also associates with the type I BMP receptor Activin RIB/ALK 4. The identification of a binding partner for VISTA proved elusive for many years until two 2019 publications identified two distinct ligands for VISTA, VSIG-3 and PSGL-1. Both ligands are expressed on T cells and blocking antibodies to VISTA have been shown to reverse immune suppression making VISTA a potential therapeutic target for cancer. 产品性质 别名Synonyms B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 Uniprot No. Q9H7M9 Source Recombinant Human B7-H5/Gi24/VISTA is expressed from HEK293with His tag at the C-terminal. It contains Phe33-Ala194. 分子量(Molecular Weight) The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 32-70 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data: Immobilized Human B7-H5, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50of 16.6ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.