Recombinant Human SIRP alpha/CD172a(His Tag)

Description :
FAQ COA 已发表文献 Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa typeI transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha, respectively; it shares 84% and 76%aa identity with human SIRP beta 1 and SIRP gamma, respectively. SIRP alpha engagement generally produces a negative regulatory signal. Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation. SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production. The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis. 产品性质 别名Synonyms CD172a, BIT, MFR, MYD1, MYD-1, P84, PTPNS1, SHPS1, SHPS-1, SIRP alpha, SIRPA Uniprot No. P78324-1 Source HEK293 Cells-derivedHuman SIRP alpha / CD172a Protein, Glu31-Arg370with His tag at the C-terminal 分子量(Molecular Weight) Approximately 38.1kDa.Due to glycosylation, the protein migrates to 45-65kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity 1.ELISAData: Immobilized Human SIRP alpha, His Tag at 0.5g/mL(100L/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50of 38.1ng/mL determined by ELISA. 2.SPRData: Anti-SIRP alpha Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200). 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.