Recombinant Human Sigle10(hFc-Avi Tag)

Description :
FAQ COA 已发表文献 Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G.Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and Bcells with some splice variants predominating in particular cell types and tissue locations. It is upregulated on eosinophils in mouse models of allergic respiratory inflammation. 产品性质 别名Synonyms SLG2, SIGLEC10, MGC126774, PRO940 Uniprot No. Q96LC7-1 Source HEK293 Cells-derivedHuman Siglec-10 Protein, Met17-Thr546 with hFc tag and Avi tagat the C-terminal 分子量(Molecular Weight) Approximately 85.8kDa.Due to glycosylation, the protein migrates to 95-110kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISAData: Immobilized Human Siglec-10 at 2g/mL(100L/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50of 14.4ng/mLdetermined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.