Recombinant Human CD3E&CD3D/CD3 epsilon & CD3 delta(His Tag)

Description :
FAQ COA 已发表文献 T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3d , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3d, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. 产品性质 别名Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3d; CD3E&CD3d; CD3 delta&CD3 epsilon Uniprot No. P07766(CD3E)&P04234(CD3d) Source Recombinant Human CD3E&CD3d/CD3 epsilon&CD3 delta Protein is expressed from HEK293 Cellswith His tag at the C-terminal. It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3d). 分子量(Molecular Weight) The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3d). Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity 1.ELISA DataImmobilized Human CD3E&CD3d at 2g/ml (100l/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50of 38ng/ml determined by ELISA. 2.ELISA DataImmobilized Human CD3E&CD3d at 2ug/ml (100l/Well). Dose response curve for Foralumab, hFc Tag with the EC50of 18.5ng/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.