Recombinant Human CD24(hFc Tag)

Description :
FAQ COA 已发表文献 CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30kDa60kDa GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associatesin ciswith Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs. Mature human CD24 shares 30%and 42% amino acid sequence identity with mouse and rat CD24, respectively. 产品性质 别名Synonyms CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043 Uniprot No. P25063 Source Recombinant Human CD24 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Ser27-Gly59. 分子量(Molecular Weight) The protein has a predicted MW of 29.9 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result. 外观(Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA DataImmobilized Human CD24, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-CD24 Antibody, hFc Tag with the EC50of 0.26g/ml determined by ELISA. 内毒素Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mLis recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.