Mouse GDF-5/BMP-14

Description :
FAQ COA 已发表文献 Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins. Mature mouseGDF-5 shares 99% aa sequence identity with both maturehuman and rat GDF-5.Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bonemorphogenesis, cell survival, and neuritogenesis. Product Properties Synonyms CDMP-1, GDF5, OS5, radotermin, SYNS2 Accession P43027 GeneID 14563 Source E.coli-derived mouse GDF-5/BMP-14 protein, Ala376-Arg495. Molecular Weight Approximately 27.2 kDa. AA Sequence APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >96% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by inducing alkaline phosphatase production of mouse ATDC5 cells is less than 1.0 g/ml, corresponding to a specific activity of > 1000 IU/mg. Fully biologically active when compared to standard. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 30% Acetonitrile and 0.1% TFA. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.