Ovine IFN-τ

Description :
FAQ COA 已发表文献 IFN-is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN- has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-/. The current investigation concerns the effect of recombinant ovine IFN-tau (rOvIFN-) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and up regulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduce the relapse rate in MS is via interference with IFN–induced MHC class II expression. IFN-was shown to down regulate IFN–induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in down regulating inflammation in the central nervous system (CNS). IFN-did not upregulate the expression of MHC class II on CVE. IFN-also inhibited the replication of Theiler’s virus in CVE. Product Properties Synonyms Interferon tau-1IFN-tau-1Trophoblast antiluteolytic proteinTrophoblast protein 1 Accession P56828 GeneID 100144750 Source E.coli-derived ovine Interferon-tau protein, Cys24-Pro195 Molecular Weight Approximately 19.9 kDa. AA Sequence CYLSRKLMLD ARENLKLLDR MNRLSPHSCL QDRKDFGLPQ EMVEGDQLQK DQAFPVLYEM LQQSFNLFYT EHSSAAWDTT LLEQLCTGLQ QQLDHLDTCR GQVMGEEDSE LGNMDPIVTV KKYFQGIYDY LQEKGYSDCA WEIVRVEMMR ALTVSTTLQK RLTKMGGDLN SP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to IFN-alpha. The specific activity determined by a viral resistance assay is no less than 1.0 107IU/mg. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.